| 质粒名称: | pRc/RSV-m CBP-HA |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 16701 |
| 存储实验室: | Richard Goodman |
|
载体骨架基本信息 |
|
| 载体名称: | pRc/RSV |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5200 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CBP,CREB binding protein |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 8000 bp |
| 基因突变: | -- |
| GenBank编号 | NP_001020603.1 NP_001020603.1 |
| 启动子 | -- |
| 标签/融合蛋白: | HA（插入件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII（如果被摧毁则未知） |
| 3' 克隆位点: | NotI（如果被摧毁则未知） |
| 5' 测序引物 | RSV promoter |
| 3' 测序引物 | BGH rev |
|
文献引用方法 |
|
| 材料和方法部分: | pRc/RSV-m CBP-HA was a gift from Richard Goodman (Addgene plasmid # 16701 ; http://n2t.net/addgene:16701 ; RRID:Addgene_16701) |
| 参考文献部分: | Phosphorylated CREB binds specifically to the nuclear protein CBP. Chrivia JC, Kwok RP, Lamb N, Hagiwara M, Montminy MR, Goodman RH. Nature. 1993 Oct 28. 365(6449):855-9. 10.1038/365855a0 PubMed 8413673 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pRc/RSV-m CBP-HA 12613 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pRc/RSV-m CBP-HA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12613)
AUTHORS Chrivia JC, Kwok RP, Lamb N, Hagiwara M, Montminy MR, Goodman RH
TITLE Phosphorylated CREB binds specifically to the nuclear protein CBP.
JOURNAL Nature. 1993 Oct 28. 365(6449):855-9.
PUBMED 8413673
REFERENCE 2 (bases 1 to 12613)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12613
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(44..63)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
promoter 340..601
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
regulatory 5513..5522
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 7988..8014
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
primer_bind complement(8058..8075)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8064..8288
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8334..8847
/direction=RIGHT
/label=M13 ori
/note="M13 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(8421..8440)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 8631..8652
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(8870..8890)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 8875..9204
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9055..9190
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9117..9136
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 9271..10065
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(9325..9344)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 9935..9954
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 10239..10373
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10276..10295)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10330..10349
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10404..10420)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10404..10420)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10417..10439)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10428..10444
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10452..10482)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10497..10518
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10687..10704)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10858..11446)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10938..10957)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11617..12477)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12240..12259
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12478..12582)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgtatctgag
241 gggactaggg tgtgtttagg cgaaaagcgg ggcttcggtt gtacgcggtt aggagtcccc
301 tcaggatata gtagtttcgc ttttgcatag ggagggggaa atgtagtctt atgcaatact
361 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
421 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
481 acagacgggt ctgacatgga ttggacgaac cactgaattc cgcattgcag agatattgta
541 tttaagtgcc tagctcgata caataaacgc catttgacca ttcaccacat tggtgtgcac
601 ctccaagctt gatatcgaat tcctgcagcc cgggggatcc agaatggccg agaacttgct
661 ggacggaccg cccaacccca aacgagccaa actcagctcg cccggcttct ccgcgaatga
721 caacacagat tttggatcat tgtttgactt ggaaaatgac cttcctgatg agctgatccc
781 caatggagaa ttaagccttt taaacagtgg gaaccttgtt ccagatgctg cgtccaaaca
841 taaacaactg tcagagcttc ttagaggagg cagcggctct agcatcaacc cagggatagg
901 caatgtgagt gccagcagcc ctgtgcaaca gggccttggt ggccaggctc aggggcagcc
961 gaacagtaca aacatggcca gcttaggtgc catgggcaag agccctctga accaaggaga
1021 ctcatcaaca cccaacctgc ccaaacaggc agccagcacc tctgggccca ctccccctgc
1081 ctcccaagca ctgaatccac aagcacaaaa gcaagtaggg ctggtgacca gtagtcctgc
1141 cacatcacag actggacctg ggatctgcat gaatgctaac ttcaaccaga cccacccagg
1201 ccttctcaat agtaactctg gccatagctt aatgaatcag gctcaacaag ggcaagctca
1261 agtcatgaat ggatctcttg gggctgctgg aagaggaagg ggagctggaa tgccctaccc
1321 tgctccagcc atgcaggggg ccacaagcag tgtgctggcg gagaccttga cacaggtttc
1381 cccacaaatg gctggccatg ctggactaaa tacagcacag gcaggaggca tgaccaagat
1441 gggaatgact ggtaccacaa gtccatttgg acaacccttt agtcaaactg gagggcagca
1501 gatgggagcc actggagtta acccccagtt agccagcaaa cagagcatgg tcaatagttt
1561 acctgctttt cctacagata tcaagaatac ttcagtcacc actgtgccaa atatgtccca
1621 gttgcaaaca tcagtgggaa ttgtacccac acaagcaatt gcaacaggcc ccacagcaga
1681 ccctgaaaaa cgcaaactga tacagcagca gctggttcta ctgcttcatg cccacaaatg
1741 tcagagacga gagcaagcaa atggagaggt tcgagcctgt tctctcccac actgtcgaac
1801 catgaaaaac gttttgaatc acatgacaca ttgtcaggct gggaaagcct gccaagttgc
1861 ccattgtgca tcttcacgac aaatcatctc tcattggaag aactgcacac gacatgactg
1921 tcctgtttgc ctccctttga aaaatgccag tgacaagcga aaccaacaaa ccatcctggg
1981 atctccagct agtggaattc aaaacacaat tggttctgtt ggtgcagggc aacagaatgc
2041 cacttcctta agtaacccaa atcccataga ccccagttcc atgcagcggg cctatgctgc
2101 tctaggactc ccctacatga accagcctca gacgcagctg cagcctcagg ttcctggcca
2161 gcaaccagca cagcctccag cccaccagca gatgaggact ctcaatgccc taggaaacaa
2221 ccccatgagt atcccagcag gaggaataac aacagatcaa cagccaccaa acttgatttc
2281 agaatcagct cttccaactt ccttgggggc taccaatcca ctgatgaatg atggttcaaa
2341 ctctggtaac attggaagcc tcagcacgat acctacagca gcgcctcctt ccagcactgg
2401 tgttcgaaaa ggctggcatg aacatgtgac tcaggaccta cggagtcatc tagtccataa
2461 actcgttcaa gccatcttcc caactccaga ccctgcagct ctgaaagatc gccgcatgga
2521 gaacctggtt gcctatgcta agaaagtgga gggagacatg tatgagtctg ctaatagcag
2581 ggatgaatac tatcatttat tagcagagaa aatctataaa atacaaaaag aactagaaga
2641 aaagcggagg tcacgtttac ataagcaagg catcctgggt aaccagccag ctttaccagc
2701 ttctggggct cagccccctg tgattccacc agcccagtct gtaagacctc caaatgggcc
2761 cctgcctttg ccagtgaatc gcatgcaggt ttctcaaggg atgaattcat ttaacccaat
2821 gtccctggga aacgtccagt tgccacaggc acccatggga cctcgtgcag cctcccctat
2881 gaaccactct gtgcagatga acagcatggc ctcagttccg ggtatggcca tttctccttc
2941 acggatgcct cagcctccaa atatgatggg cactcatgcc aacaacatta tggcccaggc
3001 acctactcag aaccagtttc tgccacagaa ccagtttcca tcatccagtg gggcaatgag
3061 tgtgaacagt gtgggcatgg ggcaaccagc agcccaggca ggtgtttcac agggtcaggt
3121 acctggagct gctctcccta accctctgaa catgctggca ccccaggcca gccagctgcc
3181 ttgcccacca gtgacacagt caccattgca cccgactcca cctcctgctt ccacagctgc
3241 tggcatgccc tctctccaac atccaacggc accaggaatg acccctcctc agccagcagc
3301 tcccactcag ccatctactc ctgtgtcatc tgggcagact cctaccccaa ctcctggctc
3361 agtgcccagc gctgcccaaa cacagagtac ccctacagtc caggcagcag cacaggctca
3421 ggtgactcca cagcctcaga ccccagtgca gccaccatct gtggctactc ctcagtcatc
3481 acagcagcaa ccaacgcctg tgcatactca gcctcctggc acaccgcttt ctcaggcagc
3541 agccagcatt gataatagag tccctactcc ctcctctgtg accagtgctg aaaccagttc
3601 ccagcagcca ggacccgatg tgcccatgct ggaaatgaag acagaggtgc agacagatga
3661 tgctgagcct gaacctactg aatccaaggg ggaacctcgg tctgagatga tggaagagga
3721 tttacaaggt tcttcccaag taaaagaaga gacagatacg acagagcaga agtcagagcc
3781 aatggaagta gaagaaaaga aacctgaagt aaaagtggaa gctaaagagg aagaagagaa
3841 cagttcgaac gacacagcct cacaatcaac atctccttcc cagccacgca aaaaaatctt
3901 taaacccgag gagctacgcc aggcacttat gccaactcta gaagcactct atcgacagga
3961 cccagagtct ttgccttttc gtcagcctgt agatcctcag ctcctaggaa tcccagatta
4021 ttttgatata gtgaagaatc ctatggacct ttctaccatc aaacgaaagc tggacacagg
4081 gcaatatcaa gaaccctggc agtatgtgga tgatgtctgg cttatgttca acaatgcgtg
4141 gctatataat cgtaaaacgt cccgtgtata taaattttgc agtaaacttg cagaggtctt
4201 tgaacaagaa attgaccctg tcatgcagtc tcttggatat tgctgtggac gaaagtatga
4261 gttctcccca cagactttgt gctgttacgg aaagcagctg tgtacaattc ctcgtgatgc
4321 agcctactac agctatcaga ataggtatca tttctgtgag aagtgtttca cagagatcca
4381 gggcgagaat gtgaccctgg gtgacgaccc ttcccaacct cagacgacaa tttccaagga
4441 tcaatttgaa aagaagaaaa atgatacctt agatcctgaa ccttttgttg actgcaaaga
4501 gtgtggccgg aagatgcatc agatttgtgt tctacactat gacatcattt ggccttcagg
4561 ttttgtgtgt gacaactgtt tgaagaaaac tggcagacct cggaaagaaa acaaattcag
4621 tgctaagagg ctgcagacca cacgattggg aaaccactta gaagacagag tgaataagtt
4681 tttgcggcgc cagaatcacc ctgaagctgg ggaggttttt gtcagagtgg tggccagctc
4741 agacaagact gtggaggtca agccgggaat gaagtcaagg tttgtggatt ctggagagat
4801 gtcggaatct ttcccatatc gtaccaaagc actctttgct tttgaggaga tcgatggagt
4861 cgatgtgtgc ttttttggga tgcatgtgca agaatacggc tctgattgcc ccccaccaaa
4921 tacaaggcgt gtatacatat cttatctgga cagtattcat ttcttccggc cccgctgcct
4981 ccggacagct gtttaccatg agatcctcat cggatatctc gagtatgtga agaaattggg
5041 gtatgtgaca ggacatattt gggcctgtcc cccaagtgaa ggagatgact atatctttca
5101 ttgccacccc cctgaccaga aaatccccaa accaaaacga ctacaggagt ggtacaagaa
5161 gatgctggac aaggcgtttg cagagaggat cattaacgac tataaggaca tcttcaaaca
5221 agcgaacgaa gacaggctca cgagtgccaa ggagttgccc tattttgaag gagatttctg
5281 gcctaatgtg ttggaagaaa gcattaagga actagaacaa gaagaagaag aaaggaaaaa
5341 agaagagagt actgcagcga gtgagactcc tgagggcagt cagggtgaca gcaaaaatgc
5401 gaagaaaaag aacaacaaga agaccaacaa aaacaaaagc agcattagcc gcgccaacaa
5461 gaagaagccc agcatgccca atgtttccaa cgacctgtcg cagaagctgt atgccaccat
5521 ggagaagcac aaggaggtat tctttgtgat tcatctgcat gctgggcctg ttatcagcac
5581 tcagcccccc atcgtggacc ctgatcctct gcttagctgt gacctcatgg atgggcgaga
5641 tgccttcctc accctggcca gagacaagca ctgggaattc tcttccttac gccgctccaa
5701 atggtccact ctgtgcatgc tggtggagct gcacacacag ggccaggacc gctttgttta
5761 tacctgcaat gagtgcaaac accatgtgga aacacgctgg cactgcactg tgtgtgagga
5821 ctatgacctt tgtatcaatt gctacaacac aaagagccac acccataaga tggtgaagtg
5881 ggggctaggc ctagatgatg agggcagcag tcagggtgag ccacagtcca agagccccca
5941 ggaatcccgg cgtctcagca tccagcgctg catccagtcc ctggtgcatg cctgccagtg
6001 tcgcaatgcc aactgctcac tgccgtcttg ccagaagatg aagcgagtcg tgcagcacac
6061 caagggctgc aagcgcaaga ctaatggagg atgcccagtg tgcaagcagc tcattgctct
6121 ttgctgctac cacgccaaac actgccaaga aaataaatgc cctgtgccct tctgcctcaa
6181 catcaaacat aagctccgcc agcagcagat ccagcatcgc ctgcagcagg ctcagctcat
6241 gcgccggcga atggcaacca tgaacacccg caatgtgcct cagcagagtt tgccttctcc
6301 tacctcagca ccacccggga ctcctacaca gcagcccagc acaccccaaa caccacagcc
6361 cccagcccag cctcagcctt cacctgttaa catgtcacca gctggcttcc ctaatgtagc
6421 ccggactcag cccccaacaa tagtgtctgc tgggaagcct accaaccagg tgccagctcc
6481 cccaccccct gcccagcccc cacctgcagc agtagaagca gcccggcaaa ttgaacgtga
6541 ggcccagcag cagcagcacc tataccgagc aaacatcaac aatggcatgc ccccaggacg
6601 tgcaggtatg gggaccccag gaagccaaat gactcctgtg ggcctgaatg tgccccgtcc
6661 caaccaagtc agtgggcctg tcatgtctag tatgccacct gggcagtggc agcaggcacc
6721 catccctcag cagcagccga tgccaggcat gcccaggcct gtaatgtcca tgcaggccca
6781 ggcagcagtg gctgggccac ggatgcccaa tgtgcagcca ccaaggagca tctcgccaag
6841 tgccctgcaa gacctgctac ggaccctaaa gtcacccagc tctcctcagc agcagcagca
6901 ggtgctgaac atccttaaat caaacccaca gctaatggca gctttcatca aacagcgcac
6961 agccaagtat gtggccaatc agcctggcat gcagccccag cccggacttc aatcccagcc
7021 tggtatgcag ccccagcctg gcatgcacca gcagcctagt ttgcaaaacc tgaacgcaat
7081 gcaagctggt gtgccacggc ctggtgtgcc tccaccacaa ccagcaatgg gaggcctgaa
7141 tccccaggga caagctctga acatcatgaa cccaggacac aaccccaaca tgacaaacat
7201 gaatccacag taccgagaaa tggtgaggag acagctgcta cagcaccagc agcagcagca
7261 gcaacagcag cagcagcagc agcaacaaca aaatagtgcc agcttggccg ggggcatggc
7321 gggacacagc cagttccagc agccacaagg acctggaggt tatgccccag ccatgcagca
7381 gcaacgcatg caacagcacc tccccatcca gggcagctcc atgggccaga tggctgctcc
7441 aatgggacaa cttggccaga tggggcagcc tgggctaggg gcagacagca cccctaatat
7501 ccagcaggcc ctgcagcaac ggattctgca gcagcagcag atgaagcaac aaattgggtc
7561 accaggccag ccgaacccca tgagccccca gcagcacatg ctctcaggac agccacaggc
7621 ctcacatctc cctggccagc agatcgccac atcccttagt aaccaggtgc gatctccagc
7681 ccctgtgcag tctccacggc cccaatccca acctccacat tccagcccgt caccacggat
7741 acaaccccag ccttcaccac accatgtttc accccagact ggttcccctc accctggact
7801 cgcagtcacc atggccagct ccatggatca gggacacctg gggaaccctg aacagagtgc
7861 aatgctcccc cagctgaata cccccaacag gagcgcactg tccagtgaac tgtccctggt
7921 tggtgatacc acgggagaca cactagaaaa gtttgtggag ggtttgaagc tggatccggg
7981 gatggcctac ccatacgacg tgcctgacta cgcctcctag cggccgctcg agcatgcatc
8041 tagagctcgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
8101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
8161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
8221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
8281 ctctatggct tctgaggcgg aaagaaccag ctggggctcg aggggggatc cccacgcgcc
8341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
8401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
8461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
8521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
8581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
8641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
8701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
8761 ttttaacaaa atattaacgc ttacaattta aatatttgct tatacaatct tcctgttttt
8821 ggggcttttc tgattatcaa ccggggtggg taccgagctc gaattctgtg gaatgtgtgt
8881 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
8941 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
9001 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
9061 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
9121 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
9181 tttggaggcc taggcttttg caaaaagctc ccgggagctt ggatatccat tttcggatct
9241 gatcaagaga caggatgagg atcgtttcgc atgattgaac aagatggatt gcacgcaggt
9301 tctccggccg cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc
9361 tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag
9421 accgacctgt ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg
9481 gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac
9541 tggctgctat tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc
9601 gagaaagtat ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc
9661 tgcccattcg accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc
9721 ggtcttgtcg atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg
9781 ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat
9841 gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc
9901 cggctgggtg tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa
9961 gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat
10021 tcgcagcgca tcgccttcta tcgccttctt gacgagttct tctgagcggg actctggggt
10081 tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg agatttcgat tccaccgccg
10141 ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg atgatcctcc
10201 agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt gcagcttata
10261 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
10321 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccgtcgac
10381 ctcgagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
10441 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
10501 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
10561 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
10621 cgctcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
10681 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
10741 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
10801 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
10861 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
10921 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
10981 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
11041 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
11101 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
11161 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
11221 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
11281 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
11341 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
11401 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
11461 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
11521 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
11581 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
11641 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
11701 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
11761 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
11821 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
11881 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
11941 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
12001 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
12061 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
12121 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
12181 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
12241 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
12301 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
12361 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
12421 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
12481 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
12541 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
12601 gccacctgac gtc
//
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